[protocols] Fwd: Kalamazoo Metagenome Assembly Question
Adina Chuang Howe
howead at msu.edu
Fri Oct 25 05:36:49 PDT 2013
Hi folks,
https://khmer-protocols.readthedocs.org/en/v0.8/metagenomics/1-quality.html
Is there a reason s2_se leftover from trimmomatic are excluded from this?
These files are generally smaller in my datasets (though I'm missing the
biological reason for this). As far as I know, these are the second pair's
adapter-trimmed and possibly now unpaired. I think they should be treated
as s1_se? i.e., quality trimmed and then concatenated with all single ends.
Cheers,
-adina
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