[protocols] Fwd: Kalamazoo Metagenome Assembly Question
C. Titus Brown
ctb at msu.edu
Tue Oct 29 19:04:50 PDT 2013
On Fri, Oct 25, 2013 at 08:36:49AM -0400, Adina Chuang Howe wrote:
> Hi folks,
>
>
> https://khmer-protocols.readthedocs.org/en/v0.8/metagenomics/1-quality.html
>
> Is there a reason s2_se leftover from trimmomatic are excluded from this?
> These files are generally smaller in my datasets (though I'm missing the
> biological reason for this). As far as I know, these are the second pair's
> adapter-trimmed and possibly now unpaired. I think they should be treated
> as s1_se? i.e., quality trimmed and then concatenated with all single ends.
No good reason. I've just never had one generated. This is on the list to
fix...
--t
--
C. Titus Brown, ctb at msu.edu
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