[ngs] some info on protocol etc for RNA extraction and bioanalyzer results

[liz wright]

That would be PureLink RNA Mini Kit.  god I hate these names.  Also did
some RNA extraction with Qiagen RNeasy kit.


On Thu, Jun 27, 2013 at 3:50 PM, liz wright <lzwright at gmail.com> wrote:

> Hi all,
>
> Dana asked me for a protocol on RNA extraction, and since I have found the
> Trizol + Purelink mRNA kit to work quite well, I thought I would pass on to
> the rest of you as well.  I am attaching a conglomeration of informative
> stuff in one pdf, including representative bioanalyzer traces.  Working
> with a tech at NYU Center for Genomics and Structural Biology, have also
> used: *Clontech SMARTerTM Ultra Low RNA Kit for Illumina Sequencing*
> http://www.illumina.com/applications/sequencing/rna/ultra-low_rna_input.ilmn
>
> If this ultra low kit interests you I have some tips about synthesis of
> cDNA from total RNA, in particular the step for adding the oligo! (just let
> me know)
>
> Best, Liz
>
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