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<a href="http://www.ncbi.nlm.nih.gov/pubmed/22113299#" title="Methods in molecular biology (Clifton, N.J.)." style="margin-top:0px;margin-right:0px;margin-bottom:0px;margin-left:0px;padding-top:0px;padding-right:0px;padding-bottom:0px;padding-left:0px;border-top-width:0px;border-right-width:0px;border-bottom-width:0px;border-left-width:0px;border-style:initial;border-color:initial;outline-width:0px;outline-style:initial;outline-color:initial;font-size:11px;font:inherit;vertical-align:baseline;color:rgb(51,51,51);border-bottom-style:initial;border-bottom-color:initial;text-decoration:underline" target="_blank">Methods Mol Biol.</a> 2012;809:535-62.</div>

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Genome wide full-length transcript analysis using 5&#39; and 3&#39; paired-<span style="margin-top:0px;margin-right:0px;margin-bottom:0px;margin-left:0px;padding-top:0px;padding-right:0px;padding-bottom:0px;padding-left:0px;border-top-width:0px;border-right-width:0px;border-bottom-width:0px;border-left-width:0px;border-style:initial;border-color:initial;outline-width:0px;outline-style:initial;outline-color:initial;font-size:18px;font:inherit;vertical-align:baseline">end</span>-tag next generation sequencing (RNA-PET).</h1>

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<a href="http://www.ncbi.nlm.nih.gov/pubmed?term=Ruan%20X%5BAuthor%5D&amp;cauthor=true&amp;cauthor_uid=22113299" style="margin-top:0px;margin-right:0px;margin-bottom:0px;margin-left:0px;padding-top:0px;padding-right:0px;padding-bottom:0px;padding-left:0px;border-top-width:0px;border-right-width:0px;border-bottom-width:0px;border-left-width:0px;border-style:initial;border-color:initial;outline-width:0px;outline-style:initial;outline-color:initial;font-size:12px;font:inherit;vertical-align:baseline;color:rgb(51,51,51);border-bottom-style:initial;border-bottom-color:initial;text-decoration:underline" target="_blank">Ruan X</a>, <a href="http://www.ncbi.nlm.nih.gov/pubmed?term=Ruan%20Y%5BAuthor%5D&amp;cauthor=true&amp;cauthor_uid=22113299" style="margin-top:0px;margin-right:0px;margin-bottom:0px;margin-left:0px;padding-top:0px;padding-right:0px;padding-bottom:0px;padding-left:0px;border-top-width:0px;border-right-width:0px;border-bottom-width:0px;border-left-width:0px;border-style:initial;border-color:initial;outline-width:0px;outline-style:initial;outline-color:initial;font-size:12px;font:inherit;vertical-align:baseline;color:rgb(51,51,51);border-bottom-style:initial;border-bottom-color:initial;text-decoration:underline" target="_blank">Ruan Y</a>.</div>
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Genome Institute of Singapore, Singapore, Singapore.</p></div><div style="margin-top:1.2em;margin-right:auto;margin-bottom:auto;margin-left:auto;padding-top:0px;padding-right:0px;padding-bottom:0px;padding-left:0px;border-top-width:0px;border-right-width:0px;border-bottom-width:0px;border-left-width:0px;border-style:initial;border-color:initial;outline-width:0px;outline-style:initial;outline-color:initial;font-size:13px;font:inherit;vertical-align:baseline">

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Abstract</h3><p style="margin-top:0px;margin-right:0px;margin-bottom:0.5em;margin-left:0px;padding-top:0px;padding-right:0px;padding-bottom:0px;padding-left:0px;border-top-width:0px;border-right-width:0px;border-bottom-width:0px;border-left-width:0px;border-style:initial;border-color:initial;outline-width:0px;outline-style:initial;outline-color:initial;font-size:13px;font:inherit;vertical-align:baseline">

RNA-PET is a paired <span style="margin-top:0px;margin-right:0px;margin-bottom:0px;margin-left:0px;padding-top:0px;padding-right:0px;padding-bottom:0px;padding-left:0px;border-top-width:0px;border-right-width:0px;border-bottom-width:0px;border-left-width:0px;border-style:initial;border-color:initial;outline-width:0px;outline-style:initial;outline-color:initial;font-size:13px;font:inherit;vertical-align:baseline">end</span> tag (PET) sequencing method for full-length mRNA transcripts analysis using the next generation sequencer platforms such as Illumina GA and SOLiD. Unlike <span style="margin-top:0px;margin-right:0px;margin-bottom:0px;margin-left:0px;padding-top:0px;padding-right:0px;padding-bottom:0px;padding-left:0px;border-top-width:0px;border-right-width:0px;border-bottom-width:0px;border-left-width:0px;border-style:initial;border-color:initial;outline-width:0px;outline-style:initial;outline-color:initial;font-size:13px;font:inherit;vertical-align:baseline">RNA-Seq</span> method that sequences randomly sheared shotgun RNA short fragments, RNA-PET captures and sequences the 5&#39; and 3&#39;<span style="margin-top:0px;margin-right:0px;margin-bottom:0px;margin-left:0px;padding-top:0px;padding-right:0px;padding-bottom:0px;padding-left:0px;border-top-width:0px;border-right-width:0px;border-bottom-width:0px;border-left-width:0px;border-style:initial;border-color:initial;outline-width:0px;outline-style:initial;outline-color:initial;font-size:13px;font:inherit;vertical-align:baseline">end</span> tags of full-length <span style="margin-top:0px;margin-right:0px;margin-bottom:0px;margin-left:0px;padding-top:0px;padding-right:0px;padding-bottom:0px;padding-left:0px;border-top-width:0px;border-right-width:0px;border-bottom-width:0px;border-left-width:0px;border-style:initial;border-color:initial;outline-width:0px;outline-style:initial;outline-color:initial;font-size:13px;font:inherit;vertical-align:baseline">cDNA</span> fragments of all expressed genes in a biological sample. When mapped to reference genome, RNA-PET sequences can demarcate the boundaries of transcription units genome-wide, in addition to its ability to quantify the transcription level of each expression genes. Furthermore, the unique feature of RNA-PET is to identify fusion transcripts. Therefore, RNA-PET has been regarded as the best PET for genome annotation (1). Here in this chapter, we describe the details of the RNA-PET protocol and discuss the critical issues.</p>

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PMID:</dt> <dd style="margin-top:0px;margin-right:0px;margin-bottom:0px;margin-left:0px;padding-top:0px;padding-right:0px;padding-bottom:0px;padding-left:0px;border-top-width:0px;border-right-width:0px;border-bottom-width:0px;border-left-width:0px;border-style:initial;border-color:initial;outline-width:0px;outline-style:initial;outline-color:initial;font-size:11px;font:inherit;vertical-align:baseline;display:inline;white-space:nowrap">

22113299</dd> <dd style="margin-top:0px;margin-right:0px;margin-bottom:0px;margin-left:0px;padding-top:0px;padding-right:0px;padding-bottom:0px;padding-left:0px;border-top-width:0px;border-right-width:0px;border-bottom-width:0px;border-left-width:0px;border-style:initial;border-color:initial;outline-width:0px;outline-style:initial;outline-color:initial;font-size:11px;font:inherit;vertical-align:baseline;display:inline;white-space:nowrap">

[PubMed - indexed for MEDLINE]</dd></dl></div></div></span>