Hi all:<br><br>
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<font size="2"><span style="line-height: 115%; font-family: "Times New Roman";"></span></font>Vaibhav Tale, a PostDoc candidate from Marquette University, will be discussing his work with "Bioaugmentation for recovery of anaerobic digesters subjected to organic overload." <br>
<br>This will take place on Wednesday (tomorrow) July 28 in PSB Room 271.<br><br>Adina<br>===========<br><br>
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<p class="MsoNormal" style="margin-bottom: 0.0001pt; text-align: center; line-height: 200%;" align="center"><b><span style="font-size: 12pt; line-height: 200%; font-family: "Times New Roman";">Seminar,
Wednesday, July 28, 9:30am, Rm 271, Plant and Soil Science Bldg</span></b></p>
<p class="MsoNormal" style="text-align: center; line-height: normal;" align="center"><b style=""><span style="font-size: 13pt; font-family: "Times New Roman";">BIOAUGMENTATION FOR RECOVERY OF ANAEROBIC
DIGESTERS SUBJECTED TO ORGANIC OVERLOAD</span></b><b style=""><span style="font-family: "Times New Roman";"></span></b></p>
<p class="MsoNormal" style="margin-bottom: 0.0001pt; text-align: center; line-height: normal;" align="center"><span style="font-size: 12pt; font-family: "Times New Roman";">Dr. Vaibhav P. Tale</span></p>
<p class="MsoNormal" style="margin-bottom: 0.0001pt; text-align: center; line-height: normal;" align="center"><span style="font-size: 12pt; font-family: "Times New Roman";">Dept of Civil and Environmental
Engineering</span></p>
<p class="MsoNormal" style="margin-bottom: 0.0001pt; text-align: center; line-height: normal;" align="center"><span style="font-size: 12pt; font-family: "Times New Roman";">Marquette University</span></p>
<p class="MsoNormal" style="margin-bottom: 0.0001pt; text-align: center; line-height: normal;" align="center"><span style="font-size: 12pt; font-family: "Times New Roman";">Post-doc candidate</span></p>
<p class="MsoNormal" style="margin-bottom: 0.0001pt; text-align: center; line-height: normal;" align="center"><span style="font-size: 12pt; font-family: "Times New Roman";"> </span></p>
<p class="MsoNormal" style="margin-bottom: 0.0001pt; text-align: justify; text-indent: 0.5in; line-height: normal;"><span style="font-size: 12pt; font-family: "Times New Roman";">Anaerobic
digester upset due to organic overload is common and methods to reduce recovery
time would be beneficial. One potential method is bioaugmentation, the addition
of an external culture for performance improvement. Methanogenic community
structure differs from digester to digester and t</span><span style="font-size: 12pt; font-family: "Times New Roman";">here may exist a relation between specific
methanogenic activity (SMA) and microbial community composition.</span><span style="font-size: 12pt; font-family: "Times New Roman";"> The research presented
herein tested whether there is a relationship between SMA and community
structure. Also, the effectiveness of bioaugmentation was tested by
hypothesizing that bioaugmenting with a methanogenic, propionate-degrading
culture acclimated to small oxygen doses will help rapid recovery of
organically overloaded digesters.</span></p>
<p class="MsoNormal" style="margin-bottom: 0.0001pt; text-align: justify; text-indent: 0.5in; line-height: normal;"><span style="font-size: 12pt; font-family: "Times New Roman";">Fourteen
different anaerobic cultures were tested for SMA and microbial community using
the <i style="">mcrA</i> gene and DGGE to establish a
relationship between SMA and community structure. The culture with the highest
SMA was enriched by feeding </span><span style="font-size: 12pt; font-family: "Times New Roman";">0.17g
propionate/L-day</span><span style="font-size: 12pt; font-family: "Times New Roman";">
and different oxygen doses. The enrichment cultures were used to bioaugment
organically overloaded anaerobic digesters. Microbial communities present in
bioaugmented, non-bioaugmented and undisturbed control digesters as well as the
bioaugmentation culture were analyzed using 16S rDNA.</span></p>
<p class="MsoNormal" style="text-align: justify; line-height: normal;"><span style="font-size: 12pt; font-family: "Times New Roman";"><span style=""> </span>A statistically significant quantitative structure-activity relationship
(QSAR) between SMA and community structure could not be established,
highlighting the difficulty in establishing activity/community structure
relationships. However, the results indicated that there may be a relation
between SMA and methanogenic community compositions studied. Enriching a
culture for 25 mgO<sub>2</sub>/L-day increased its SMA by 29.7%, but higher
oxygen doses yielded lower SMA values. Bioaugmentation with this enrichment culture
reduced the time required for upset digester effluent to decrease below 1000 mg
SCOD/L by 114 days (11.4 SRTs) and the time required to reach 25 mLCH<sub>4</sub>/day
by 37 days (3.7 SRTs) respectively. Bioaugmented digesters consistently
produced lower effluent SCOD and more methane than non-bioaugmented digesters.
Bioaugmentation is a promising approach for speeding up recovery of organically
overloaded digesters. Bacterial and archaeal communities of the bioaugmented
and undisturbed control digesters had similar phylogenetic tree structures
(p>0.3), whereas the tree structures of non-bioaugmented and undisturbed
control digesters differed significantly (p<0.01). Bioaugmentation helped
restore the microbial communities of overloaded digesters to their original undisturbed
state. Higher relative abundance of clones related to </span><i><span style="font-size: 12pt; font-family: "Times New Roman";">Methanospirillum hungatei</span></i><span style="font-size: 12pt; font-family: "Times New Roman";"> may
have caused better performance of bioaugmented </span><span style="font-size: 12pt; font-family: "Times New Roman";">digesters.</span></p>
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