Hi all:<br><br>Titus has another engagement on Wed so I will be presenting the following paper:<br><br><h1>Metagenomic Sequencing of an <i>In Vitro</i>-Simulated Microbial Community</h1><br><p class="authors"><span rel="dc:creator"><span>Jenna L. Morgan</span></span>^{<a href="http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0010209#aff1">1</a>}^,^{<a href="http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0010209#aff2">2</a>}^,^{<a href="http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0010209#aff3">3</a>}, <span rel="dc:creator"><span>Aaron E. Darling</span></span>^{<a href="http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0010209#aff1">1</a>}^,^{<a href="http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0010209#aff2">2</a>}, <span rel="dc:creator"><span>Jonathan A. Eisen</span></span>^{<a href="http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0010209#aff1">1</a>}^,^{<a href="http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0010209#aff2">2</a>}^,^{<a href="http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0010209#aff3">3</a>}^{<a href="http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0010209#cor1" class="fnoteref">*</a>}</p>
<br>We describe a new data resource for measuring the accuracy of metagenomic binning methods, created by <i>in vitro</i>-simulation of a metagenomic community. Our <i>in vitro</i> simulation can be used to complement previous <i>in silico</i>
benchmark studies. In constructing a synthetic community and sequencing
its metagenome, we encountered several sources of observation bias that
likely affect most metagenomic experiments to date and present
challenges for comparative metagenomic studies. DNA preparation methods
have a particularly profound effect in our study, implying that samples
prepared with different protocols are not suitable for comparative
metagenomics.<br>