[metagenomics-jclub] up-coming metagenomics club (Nov 2nd)

[Adina Chuang Howe]

Hope to see you on wednesday for a presentation from Fan Yang from the
Marsh lab.  See below.

Adina
---------- Forwarded message ----------
From: "Fan Yang" <snisiarc at gmail.com>
Date: Oct 30, 2011 8:08 PM
Subject: Fwd: up-coming metagenomics club (Nov 2nd)
To: "Adina Chuang Howe" <adina.chuang at gmail.com>



-------- Original Message --------  Subject: up-coming metagenomics club
(Nov 2nd)  Date: Sun, 30 Oct 2011 20:06:50 -0400  From: Fan Yang
<snisiarc at gmail.com> <snisiarc at gmail.com>  To:
metagenomics-jclub at lists.idyll.org

 Hi everyone,

I'll be presenting one of the side projects (comparative genomics) I have
been working on for the up-coming metagenomics journal club. Please see
abstract beneath. Look forward to see you in PSSB 271 at noon on the
November 2nd.

Thanks.

Fan

==============================================================
Abstract:

*Ralstonia pickettii *isolated from copper contaminated lake sediment are
adapted to high levels of copper after 100 years of selective pressure. Two
*R. pickettii *strains (12D and 12J) were selected for the studies reported
herein due to their distinct differences in genomic structure, different
metal resistance patterns and carriage of a filamentous phage. Copper
sequestration studies revealed that these strains could bind up to 27.44
(12D) and 38.19 (12J) mg copper per g dry weight of cells. The
transcriptional response of *R. pickettii *12D and 12J grown in the
presence and absence of copper showed that transcripts encoding for copper
resistance, heavy metal efflux pumps and heavy metal translocating P-type
ATPase were abundant in both copper grown *R. pickettii *12D and 12J cells.
We also observed that a large amount of genes represents signal peptides
were highly expressed under copper supplemented growth condition. Recent
completion of the genome sequences revealed that an approximately 220 KB
region enriched with metal resistance and transporter genes was found in
the large chromosome 1 of strain 12J but as a plasmid in strain 12D. In
addition, comparative sequence analysis showed that several genes might
have been derived from horizontal transfer. Hence, adaptation of* R.
pickettii* to high concentrations of metal appears due to robust gene
duplication and importation of several types of resistance determinants.
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