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<p class="x_MsoNormal">Dear Titus,</p>
<p class="x_MsoNormal">Thanks for the prompt reply.</p>
<p class="x_MsoNormal">Tried what you suggested but sadly it did not solve it.</p>
<p class="x_MsoNormal">Any other suggestion?</p>
<p class="x_MsoNormal">Regards,</p>
<p class="x_MsoNormal">Bogdan</p>
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<p class="x_MsoNormal" style="border:none; padding:0in"><b>From: </b><a href="mailto:ctbrown@ucdavis.edu">C. Titus Brown</a><br>
<b>Sent: </b>Wednesday, December 7, 2016 3:32 PM<br>
<b>To: </b><a href="mailto:bbintu@fas.harvard.edu">Bintu, Bogdan</a><br>
<b>Cc: </b><a href="mailto:khmer@lists.idyll.org">khmer@lists.idyll.org</a><br>
<b>Subject: </b>Re: [khmer] Unique reverse-complement</p>
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<div class="PlainText">On Wed, Dec 07, 2016 at 08:18:31PM +0000, Bintu, Bogdan wrote:<br>
> Dear Dr. Brown,<br>
> <br>
> I am a graduate student using your khmer package.<br>
> I am trying to construct a khmer.Countgraph which is not degenerate between a sequence and its reverse complement.<br>
> More explicitly, in python when running:<br>
> <br>
> import Khmer<br>
> ht = khmer.Countgraph(17,10,1)<br>
> ht.consume('GGCTTGCTCCAGCCATG')<br>
> ht.get('CATGGCTGGAGCAAGCC')<br>
> <br>
> I would like the last line to return 0.<br>
> <br>
> As suggested in a different thread I tired modifying NO_UNIQUE_RC=0 to NO_UNIQUE_RC=1 in lib/Makefile and then run python setup.py install.<br>
> But this did not solve my issue.<br>
> <br>
> Any help would be appreciated.<br>
<br>
Hi Bogdan,<br>
<br>
thanks for the email!<br>
<br>
Can you try doing:<br>
<br>
make clean<br>
python setup.py install<br>
<br>
after the modification? That should work.<br>
<br>
thanks,<br>
--titus<br>
-- <br>
C. Titus Brown, ctbrown@ucdavis.edu<br>
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