<div dir="ltr"><br><ol class=""><li>Precisely describe what you are trying to do. Reread it from the
perspective of someone else trying to reproduce your task.</li><li>Copy-and-paste the exact command that is causing the problem. Include the
steps you performed leading up to the issue.</li><li>Include the complete error message; if it is large, include a link to a
file.</li></ol><p><br></p><p>Linux 3.19.0-15-generic x86_64 (Virtualbox guest on a Windows 7 host)<br></p><p>Python 2.7.9</p><p>Khmer1.1</p><p>Hello,</p><p>I'm trying to work through trimming and normalizing a data set and I receive the following error from <b>interleave-reads.py</b> after running Trimmomatic. I have successfully run this data set through on another machine as-is and with the same commands but now I receive this error. <br></p><p><br></p><p>Input Read Pairs: 3995957 Both Surviving: 838881 (20.99%) Forward Only Surviving: 3157067 (79.01%) Reverse Only Surviving: 0 (0.00%) Dropped: 9 (0.00%)<br>TrimmomaticPE: Completed successfully<br><br>|| This is the script 'interleave-reads.py' in khmer.<br>|| You are running khmer version 1.1<br>|| You are also using screed version 0.9<br>||<br>|| If you use this script in a publication, please cite EACH of the following:<br>||<br>|| * MR Crusoe et al., 2014. doi: 10.6084/m9.figshare.979190<br>||<br>|| Please see the CITATION file for details.<br><br>Interleaving:<br> s1_pe<br> s2_pe<br>... 0 pairs<br>Traceback (most recent call last):<br> File "/usr/local/share/khmer/scripts/interleave-reads.py", line 124, in <module><br> main()<br> File "/usr/local/share/khmer/scripts/interleave-reads.py", line 115, in main<br> "This doesn't look like paired data! %s %s" % (name1, name2)<br>AssertionError: This doesn't look like paired data! M00366:65:000000000-ABGLH:1:1101:10774:1303 1:N:0:6/1 M00366:65:000000000-ABGLH:1:1101:10774:1303 2:N:0:6/2<br></p><p><br></p><p>Thanks!</p><p>Dacotah<br></p></div>