<div dir="ltr"><div><div><div><div>Hi all,<br><br></div>I am seeing some strange behavior running normalize-by-median.py. The program seemed to complete successfully after 30-45 minutes, but then it just hung there. It's now been at least 90 minutes and it's continuing to hang. The output file seems to contain all the data except the last record, and the second-to-last record is cut off.<br><br><span style="font-family:courier new,monospace">(khmer-env)[standage@bggnomic qc] tail SRR494178_int.fastq.keep<br>+<br>GBGED>>E##################################################################################<br>@SRR494178.12090255/1<br>TCGAGGACNACCTTTTGACCCTTCTGCAACCTTTGAATTTCAGACATCAAACTCTCCCTCTGTCGTGTCTCCNNCAATGATGGGTCGGGC<br>+<br>IIIIIGGG#GGGGGGIIIIIIIIIIIIIIIIGIHIIIIIGIIIIIIIIIIIIIHIIIIHIEGHHIFIHII=?##?;9>>;IGBFFGBD8G<br>@SRR494178.12090255/2<br>GATTCCGTCACCGAGGAGTATCCGTTGCCGAGGTTGTGCGTCTGTCGAACCTGGCCGTTCTTTTTGACCGTGTAGGTGCCGCCGTTGATC<br>+<br>IIIIIIHIIIIIIIIIBIHHIIIGIIIIIII(khmer-env)[standage@bggnomic qc]</span><br><br></div>Any ideas as to what could be causing this?<br><br></div>Thanks,<br></div>Daniel<br><br>PS.<br><ul><li>OS: Fedora 20 with lots o RAM (100s of GB)</li><li>Command: <span style="font-family:courier new,monospace">normalize-by-median.py -k 17 -p -N 4 -x 8e9 SRR494178_int.fastq</span></li><li>Data: <a href="http://www.ncbi.nlm.nih.gov/sra/?term=SRR494178">http://www.ncbi.nlm.nih.gov/sra/?term=SRR494178</a><br></li></ul><div><div><div><div><div><div><div dir="ltr"><br>--<br>Daniel S. Standage<br>Ph.D. Candidate<br>Computational Genome Science Laboratory<br>Indiana University<br></div></div>
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