<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=Windows-1252">
</head>
<body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">
<div>
<div>Personally, I interleave the reads and run them together for all the analyses, then split them later (after filtering, etc). &nbsp;But it would be interesting to hear of other options…</div>
<div><br>
</div>
<div>chris</div>
<div><br>
</div>
<div>On Oct 16, 2013, at 3:54 PM, Nacho Caballero &lt;<a href="mailto:nachocab@gmail.com">nachocab@gmail.com</a>&gt;</div>
<div>&nbsp;wrote:</div>
<br class="Apple-interchange-newline">
<blockquote type="cite">
<div dir="ltr">
<div class="markdown-here-wrapper" id="markdown-here-wrapper-801403" style="font-size:1em;font-family:Helvetica,arial,freesans,clean,sans-serif;color:rgb(34,34,34);background-color:rgb(255,255,255);border:none;line-height:1.2">
<p style="margin:1em 0px">I’m following the STAMPS <a href="http://ged.msu.edu/angus/2013-hmp-assembly-webinar/exploring-stamps-data.html" style="font-weight:bold;color:rgb(17,85,204);text-decoration:underline">
tutorial</a> and I created the bloom filter for my two paired end files </p>
<pre style="font-size:0.85em;font-family:Consolas,Inconsolata,Courier,monospace;font-size:1em;line-height:1.2em;overflow:auto;margin:1em 0px"><code style="font-size:0.85em;font-family:Consolas,Inconsolata,Courier,monospace;margin:0px 0.15em;padding:0px 0.3em;white-space:nowrap;border:1px solid rgb(234,234,234);background-color:rgb(248,248,248);border-top-left-radius:3px;border-top-right-radius:3px;border-bottom-right-radius:3px;border-bottom-left-radius:3px;display:inline;white-space:pre;border-top-left-radius:3px;border-top-right-radius:3px;border-bottom-right-radius:3px;border-bottom-left-radius:3px;border:1px solid rgb(204,204,204);padding:0.5em 0.7em;display:block;padding:0.5em;color:rgb(51,51,51);background-color:rgb(248,248,255);background-repeat:initial initial">python load-into-counting.py -x 1e8 -k 20 <a href="http://sample.kh/">sample.kh</a> sample_1.fastq sample_2.fastq</code></pre>
<p style="margin:1em 0px">Now I’m trying to generate the distribution, but there is no -p option, how should I do it?</p>
<pre style="font-size:0.85em;font-family:Consolas,Inconsolata,Courier,monospace;font-size:1em;line-height:1.2em;overflow:auto;margin:1em 0px"><code style="font-size:0.85em;font-family:Consolas,Inconsolata,Courier,monospace;margin:0px 0.15em;padding:0px 0.3em;white-space:nowrap;border:1px solid rgb(234,234,234);background-color:rgb(248,248,248);border-top-left-radius:3px;border-top-right-radius:3px;border-bottom-right-radius:3px;border-bottom-left-radius:3px;display:inline;white-space:pre;border-top-left-radius:3px;border-top-right-radius:3px;border-bottom-right-radius:3px;border-bottom-left-radius:3px;border:1px solid rgb(204,204,204);padding:0.5em 0.7em;display:block;padding:0.5em;color:rgb(51,51,51);background-color:rgb(248,248,255);background-repeat:initial initial">python abundance-dist.py <a href="http://sample.kh/">sample.kh</a> ??.fastq sample.hist</code></pre>
</div>
<div></div>
</div>
_______________________________________________<br>
khmer mailing list<br>
<a href="mailto:khmer@lists.idyll.org">khmer@lists.idyll.org</a><br>
http://lists.idyll.org/listinfo/khmer<br>
</blockquote>
</div>
<br>
</body>
</html>