<div dir="ltr"><div><div>Hi Daniel,<br><br></div>I had the same error recently and learned that the maximum boundary of for khmer is 32. I was told that a relaxation of this limitation is in the pipeline.<br><br>Best,<br><br>
</div>Karl<br></div><div class="gmail_extra"><br><br><div class="gmail_quote">On Mon, Aug 5, 2013 at 8:38 AM, cy_jiang <span dir="ltr"><<a href="mailto:cy_jiang@126.com" target="_blank">cy_jiang@126.com</a>></span> wrote:<br>
<blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex"><div style="line-height:1.7;font-size:14px;font-family:arial"><div><div><span style="font-size:14px;line-height:1.7">Hi all,</span><div style="line-height:1.7;font-size:14px;font-family:arial">
<br></div><div style="line-height:1.7;font-size:14px;font-family:arial">I am wondering if I can use diginorm to remove identical reads from my dataset.<font color="#333333" face="Arial, 宋体, 微软雅黑"><span style="line-height:24px"><b> </b></span></font></div>
<div style="line-height:1.7;font-size:14px;font-family:arial"><font color="#333333" face="Arial, 宋体, 微软雅黑"><span style="line-height:24px"><b><br></b></span></font></div><div style="line-height:1.7;font-size:14px;font-family:arial">
<font color="#333333" face="Arial, 宋体, 微软雅黑"><span style="line-height:24px">Precisely, I got paired-end reads of length 100bp. I first interleaved the reads into one file, then ran diginorm with -k 100 -C 1 -x 7.2e9 -N 4. Then the following information prompted up:</span></font></div>
<div style="line-height:1.7;font-size:14px;font-family:arial"><font color="#333333" face="Arial, 宋体, 微软雅黑"><span style="line-height:24px">python: ktable.cc:21: khmer::HashIntoType khmer::_hash(const char*, khmer::WordLength, khmer::HashIntoType&, khmer::HashIntoType&): Assertion `k <= sizeof(HashIntoType)*4' failed.</span></font></div>
<div><font style="font-size:14px" color="#333333" face="Arial, 宋体, 微软雅黑"><span style="line-height:24px">What did this exactly mean? Is there anything I can do to </span></font><font color="#333333" face="Arial, 宋体, 微软雅黑"><span style="line-height:24px">achieve the goal?</span></font></div>
</div></div><div><font color="#333333" face="Arial, 宋体, 微软雅黑"><span style="line-height:24px"><br></span></font></div><div><font color="#333333" face="Arial, 宋体, 微软雅黑"><span style="line-height:24px">Thanks in advance!</span></font></div>
<div><font color="#333333" face="Arial, 宋体, 微软雅黑"><span style="line-height:24px"><br></span></font></div><div><font color="#333333" face="Arial, 宋体, 微软雅黑"><span style="line-height:24px">Daniel</span></font></div></div><br>
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<br></blockquote></div><br></div>