[khmer] question re: trimmomatic/fastx
C. Titus Brown
ctb at msu.edu
Sun Feb 16 20:51:55 PST 2014
On Thu, Feb 13, 2014 at 04:09:51PM +0000, Fields, Christopher J wrote:
> Apologies in advanced if this is known, but I couldn?t find the answer on the list. Is there any reason behind why trimmomatic is being using only for adaptor trimming, whereas fastx is used for quality trimming? We?ve usually done this in a single trimmomatic run, something like:
> java -jar /usr/local/bin/trimmomatic-0.30.jar PE ../SRR492065_?.fastq.gz s1_pe s1_se s2_pe s2_se \
> ILLUMINACLIP:/usr/local/share/adapters/TruSeq3-PE.fa:2:30:10 \
> TRAILING:30 \
> LEADING:30 \
No reason, just what worked for us a few times.
Well, OK, I hate the Trimmomatic command line syntax... :) Would be happy
to move away from trimmomatic, frankly.
C. Titus Brown, ctb at msu.edu
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