[khmer] Using khmer on paired-end Illumina data set

Mamoon Rashid rashidmamoon at gmail.com
Tue Nov 5 02:53:52 PST 2013


Dear khmer users,
Please let me know the easy way to reduce my dataset (13.9 m PE reads)
which shows a lot of duplicates in the FastQC plots (attached). This
resulted into very fragmented assembly using velvet.
Any suggestions are most welcome.
Best regards
Mamoon


-- 
*Mamoon Rashid, PhD*
Post-Doctoral Fellow
Marine Microbial Ecology Lab
Red Sea Research Center
4700 King Abdullah University of Science and Technology (KAUST)
Room 2217-WS03, Ibn Al-Haytham Building (2)
4700 *King* *Abdullah* *University* *of* *Science* *and* *Technology*
Thuwal 23955-6900, Kingdom of Saudi Arabia
Office: +966 (0) 2 808-2671
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://lists.idyll.org/pipermail/khmer/attachments/20131105/617768c1/attachment.htm>
-------------- next part --------------
A non-text attachment was scrubbed...
Name: si14-read1-duplication.png
Type: image/png
Size: 17982 bytes
Desc: not available
URL: <http://lists.idyll.org/pipermail/khmer/attachments/20131105/617768c1/attachment-0004.png>
-------------- next part --------------
A non-text attachment was scrubbed...
Name: si14-read2-duplication.png
Type: image/png
Size: 17321 bytes
Desc: not available
URL: <http://lists.idyll.org/pipermail/khmer/attachments/20131105/617768c1/attachment-0005.png>


More information about the khmer mailing list