<html><body><div style="color:#000; background-color:#fff; font-family:times new roman, new york, times, serif;font-size:12pt"><div><span><h3 style="padding-top: 0px; padding-right: 0px; padding-bottom: 7px; padding-left: 0px; margin-top: 1.5em; margin-right: 0px; margin-bottom: 0.5em; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 1px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-size: 1.4em; font-family: Verdana, Arial, Helvetica, sans-serif; vertical-align: baseline; color: rgb(38, 115, 186); line-height: 1.2em; clear: both; border-bottom-style: solid; border-bottom-color: rgb(211, 209, 209); text-align: left; "><br></h3><div><br></div><div><br></div><div>Hey all,</div><div><br></div><div>I am planning on presenting at 11:am Tuesday the 8th.</div><div><br></div><div>-phil</div><h3
style="padding-top: 0px; padding-right: 0px; padding-bottom: 7px; padding-left: 0px; margin-top: 1.5em; margin-right: 0px; margin-bottom: 0.5em; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 1px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-size: 1.4em; font-family: Verdana, Arial, Helvetica, sans-serif; vertical-align: baseline; color: rgb(38, 115, 186); line-height: 1.2em; clear: both; border-bottom-style: solid; border-bottom-color: rgb(211, 209, 209); text-align: left; "><h1 style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-size: 1.5em;
vertical-align: baseline; line-height: 1.2em; clear: both; border-top-style: none; border-right-style: none; border-bottom-style: none; border-left-style: none; border-width: initial; ">Validation of rice genome sequence by optical mapping</h1><div class="multipleins" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-weight: normal; font-size: 11px; vertical-align: baseline; color: rgb(0, 0, 0); line-height: 16px; "><div class="authors" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0.2em; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px;
border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-size: 1em; font-family: inherit; vertical-align: baseline; overflow-x: visible; overflow-y: visible; clear: both; line-height: 1.3em; "><strong style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; ">Shiguo Zhou</strong><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px;
margin-left: 0px; "><a href="http://www.biomedcentral.com/1471-2164/8/278/#ins1" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; color: rgb(38, 115, 186); ">1</a>,</sup><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://www.biomedcentral.com/1471-2164/8/278/#ins2" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left:
0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; color: rgb(38, 115, 186); ">2</a>,</sup><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://www.biomedcentral.com/1471-2164/8/278/#ins3" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color:
initial; font-style: inherit; font-family: inherit; vertical-align: baseline; color: rgb(38, 115, 186); ">3</a></sup>, <strong style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; ">Michael C Bechner</strong><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://www.biomedcentral.com/1471-2164/8/278/#ins1" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right:
0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; color: rgb(38, 115, 186); ">1</a>,</sup><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://www.biomedcentral.com/1471-2164/8/278/#ins2" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px;
outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; color: rgb(38, 115, 186); ">2</a>,</sup><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://www.biomedcentral.com/1471-2164/8/278/#ins3" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; color: rgb(38, 115, 186); ">3</a></sup>, <strong style="padding-top: 0px; padding-right: 0px; padding-bottom:
0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; ">Michael Place</strong><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://www.biomedcentral.com/1471-2164/8/278/#ins3" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial;
border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; color: rgb(38, 115, 186); ">3</a></sup>, <strong style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; ">Chris P Churas</strong><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://www.biomedcentral.com/1471-2164/8/278/#ins1" style="padding-top: 0px;
padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; color: rgb(38, 115, 186); ">1</a>,</sup><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://www.biomedcentral.com/1471-2164/8/278/#ins2" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px;
border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; color: rgb(38, 115, 186); ">2</a>,</sup><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://www.biomedcentral.com/1471-2164/8/278/#ins3" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; color: rgb(38, 115, 186);
">3</a></sup>, <strong style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; ">Louise Pape</strong><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://www.biomedcentral.com/1471-2164/8/278/#ins1" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px;
border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; color: rgb(38, 115, 186); ">1</a>,</sup><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://www.biomedcentral.com/1471-2164/8/278/#ins2" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit;
vertical-align: baseline; color: rgb(38, 115, 186); ">2</a>,</sup><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://www.biomedcentral.com/1471-2164/8/278/#ins3" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; color: rgb(38, 115, 186); ">3</a></sup>, <strong style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left:
0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; ">Sally A Leong</strong><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://www.biomedcentral.com/1471-2164/8/278/#ins4" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style:
inherit; font-family: inherit; vertical-align: baseline; color: rgb(38, 115, 186); ">4</a></sup>, <strong style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; ">Rod Runnheim</strong><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://www.biomedcentral.com/1471-2164/8/278/#ins1" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px;
margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; color: rgb(38, 115, 186); ">1</a>,</sup><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://www.biomedcentral.com/1471-2164/8/278/#ins2" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial;
outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; color: rgb(38, 115, 186); ">2</a>,</sup><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://www.biomedcentral.com/1471-2164/8/278/#ins3" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; color: rgb(38, 115, 186); ">3</a></sup>, <strong style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px;
margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; ">Dan K Forrest</strong><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://www.biomedcentral.com/1471-2164/8/278/#ins1" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial;
outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; color: rgb(38, 115, 186); ">1</a>,</sup><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://www.biomedcentral.com/1471-2164/8/278/#ins2" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; color: rgb(38, 115, 186); ">2</a>,</sup><sup style="padding-top: 0px; padding-right: 0.2em;
padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://www.biomedcentral.com/1471-2164/8/278/#ins3" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; color: rgb(38, 115, 186); ">3</a></sup>, <strong style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style:
initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; ">Steve Goldstein</strong><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://www.biomedcentral.com/1471-2164/8/278/#ins1" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; color: rgb(38, 115, 186); ">1</a>,</sup><sup
style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://www.biomedcentral.com/1471-2164/8/278/#ins2" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; color: rgb(38, 115, 186); ">2</a>,</sup><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://www.biomedcentral.com/1471-2164/8/278/#ins3"
style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; color: rgb(38, 115, 186); ">3</a></sup>, <strong style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit;
vertical-align: baseline; ">Miron Livny</strong><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://www.biomedcentral.com/1471-2164/8/278/#ins5" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; color: rgb(38, 115, 186); ">5</a></sup> and <strong style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px;
border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; ">David C Schwartz</strong><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://www.biomedcentral.com/1471-2164/8/278/#ins1" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style:
inherit; font-family: inherit; vertical-align: baseline; color: rgb(38, 115, 186); ">1</a>,</sup><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><a href="http://www.biomedcentral.com/1471-2164/8/278/#ins2" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; color: rgb(38, 115, 186); ">2</a>,</sup><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px;
margin-bottom: 0px; margin-left: 0px; "><a href="http://www.biomedcentral.com/1471-2164/8/278/#ins3" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-family: inherit; vertical-align: baseline; color: rgb(38, 115, 186); ">3</a></sup><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; ">*</sup></div><div class="authors" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0.2em; border-top-width: 0px; border-right-width: 0px;
border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-size: 1em; font-family: inherit; vertical-align: baseline; overflow-x: visible; overflow-y: visible; clear: both; line-height: 1.3em; "><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><br></sup></div><div class="authors" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0.2em; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-size: 1em; font-family: inherit; vertical-align:
baseline; overflow-x: visible; overflow-y: visible; clear: both; line-height: 1.3em; "><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><br></sup></div><div class="authors" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0.2em; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-size: 1em; font-family: inherit; vertical-align: baseline; overflow-x: visible; overflow-y: visible; clear: both; line-height: 1.3em; "><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px;
"><br></sup></div><div class="authors" style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0.2em; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-style: inherit; font-size: 1em; font-family: inherit; vertical-align: baseline; overflow-x: visible; overflow-y: visible; clear: both; line-height: 1.3em; "><sup style="padding-top: 0px; padding-right: 0.2em; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; "><br></sup></div></div></h3><h3 style="padding-top: 0px; padding-right: 0px; padding-bottom: 7px; padding-left: 0px; margin-top: 1.5em; margin-right: 0px; margin-bottom: 0.5em; margin-left: 0px; border-top-width: 0px; border-right-width: 0px;
border-bottom-width: 1px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-size: 1.4em; font-family: Verdana, Arial, Helvetica, sans-serif; vertical-align: baseline; color: rgb(38, 115, 186); line-height: 1.2em; clear: both; border-bottom-style: solid; border-bottom-color: rgb(211, 209, 209); text-align: left; "><a rel="nofollow" target="_blank" href="http://www.biomedcentral.com/1471-2164/8/278" style="color: rgb(35, 71, 134); outline-width: 0px; outline-style: initial; outline-color: initial; font-family: 'times new roman', 'new york', times, serif; font-size: 16px; font-weight: normal; line-height: normal; text-align: -webkit-auto; ">http://www.biomedcentral.com/1471-2164/8/278</a> </h3><h3 style="padding-top: 0px; padding-right: 0px; padding-bottom: 7px; padding-left: 0px; margin-top: 1.5em; margin-right: 0px; margin-bottom:
0.5em; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 1px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-size: 1.4em; font-family: Verdana, Arial, Helvetica, sans-serif; vertical-align: baseline; color: rgb(38, 115, 186); line-height: 1.2em; clear: both; border-bottom-style: solid; border-bottom-color: rgb(211, 209, 209); text-align: left; "><br></h3><h3 style="padding-top: 0px; padding-right: 0px; padding-bottom: 7px; padding-left: 0px; margin-top: 1.5em; margin-right: 0px; margin-bottom: 0.5em; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 1px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-size: 1.4em; font-family: Verdana, Arial,
Helvetica, sans-serif; vertical-align: baseline; color: rgb(38, 115, 186); line-height: 1.2em; clear: both; border-bottom-style: solid; border-bottom-color: rgb(211, 209, 209); text-align: left; ">Abstract</h3><h4 style="padding-top: 4px; padding-right: 0px; padding-bottom: 4px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-size: 1.3em; font-family: Verdana, Arial, Helvetica, sans-serif; vertical-align: baseline; color: rgb(78, 78, 78); clear: both; border-top-style: none; border-right-style: none; border-bottom-style: none; border-left-style: none; border-width: initial; line-height: 1.2em; text-align: left; ">Background</h4><div style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-bottom: 1em; border-top-width: 0px;
border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-size: 11px; font-family: Verdana, Arial, Helvetica, sans-serif; vertical-align: baseline; overflow-x: visible; overflow-y: visible; clear: both; line-height: 17px; text-align: left; ">Rice feeds much of the world, and possesses the simplest genome analyzed to date within the grass family, making it an economically relevant model system for other cereal crops. Although the rice genome is sequenced, validation and gap closing efforts require purely independent means for accurate finishing of sequence build data.</div><h4 style="padding-top: 4px; padding-right: 0px; padding-bottom: 4px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-style: initial; border-color: initial; border-image:
initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-size: 1.3em; font-family: Verdana, Arial, Helvetica, sans-serif; vertical-align: baseline; color: rgb(78, 78, 78); clear: both; border-top-style: none; border-right-style: none; border-bottom-style: none; border-left-style: none; border-width: initial; line-height: 1.2em; text-align: left; ">Results</h4><div style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-bottom: 1em; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-size: 11px; font-family: Verdana, Arial, Helvetica, sans-serif; vertical-align: baseline; overflow-x: visible; overflow-y: visible; clear: both; line-height: 17px; text-align: left; ">To facilitate ongoing sequencing finishing and
validation efforts, we have constructed a whole-genome SwaI optical restriction map of the rice genome. The physical map consists of 14 contigs, covering 12 chromosomes, with a total genome size of 382.17 Mb; this value is about 11% smaller than original estimates. 9 of the 14 optical map contigs are without gaps, covering chromosomes 1, 2, 3, 4, 5, 7, 8 10, and 12 in their entirety – including centromeres and telomeres. Alignments between optical and <em style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-family: inherit; vertical-align: baseline; ">in silico </em>restriction maps constructed from
IRGSP (International Rice Genome Sequencing Project) and TIGR (The Institute for Genomic Research) genome sequence sources are comprehensive and informative, evidenced by map coverage across virtually all published gaps, discovery of new ones, and characterization of sequence misassemblies; all totalling ~14 Mb. Furthermore, since optical maps are ordered restriction maps, identified discordances are pinpointed on a reliable physical scaffold providing an independent resource for closure of gaps and rectification of misassemblies.</div><h4 style="padding-top: 4px; padding-right: 0px; padding-bottom: 4px; padding-left: 0px; margin-top: 0px; margin-right: 0px; margin-bottom: 0px; margin-left: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-size: 1.3em; font-family: Verdana, Arial, Helvetica, sans-serif; vertical-align: baseline; color: rgb(78, 78, 78);
clear: both; border-top-style: none; border-right-style: none; border-bottom-style: none; border-left-style: none; border-width: initial; line-height: 1.2em; text-align: left; ">Conclusion</h4><div style="padding-top: 0px; padding-right: 0px; padding-bottom: 0px; padding-left: 0px; margin-bottom: 1em; border-top-width: 0px; border-right-width: 0px; border-bottom-width: 0px; border-left-width: 0px; border-style: initial; border-color: initial; border-image: initial; outline-width: 0px; outline-style: initial; outline-color: initial; font-size: 11px; font-family: Verdana, Arial, Helvetica, sans-serif; vertical-align: baseline; overflow-x: visible; overflow-y: visible; clear: both; line-height: 17px; text-align: left; ">Analysis of sequence and optical mapping data effectively validates genome sequence assemblies constructed from large, repeat-rich genomes. Given this conclusion we envision new applications of such single molecule analysis that will merge
advantages offered by high-resolution optical maps with inexpensive, but short sequence reads generated by emerging sequencing platforms. Lastly, map construction techniques presented here points the way to new types of comparative genome analysis that would focus on discernment of structural differences revealed by optical maps constructed from a broad range of rice subspecies and varieties.</div></span></div><div> </div><div>Philip Trosko<br><br></div><div>cell phone:517-898-1780<br>home phone:517-349-0449<br>prtrosko@yahoo.com</div></div></body></html>